The mouse monoclonal antibody clone ICRF44 binds to human CD11b, also known as alpha M, α-M, ITGAM/CR3A, or Mac-1. This 165-170 kDa cell surface glycoprotein is part of a family of integrin α-receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g., fibrinogen (cell-matrix). In addition, integrin α's are active signaling receptors that recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin α-receptors consist of distinct combinations of integrin α-chains, which are differentially expressed. integrin alpha M (CD11b) assembles with integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3, and ICAM-4 on endothelial cells and can also facilitate the removal of iC3b-bearing foreign cells. CD11b is expressed on many cell types, including myeloid-lineage cells such as monocytes/macrophages, neutrophils, eosinophils, and basophils, and in lymphoid cells such as NK cells, peritoneal B-1 cells, Memory B cells, and microglia.

PRODUCT DETAILS

Clone: ICRF44

Specificity: CD11b

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Macaque, Pig

Format: cFluor^® BYG750

Isotype: IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
3. Marsik C, et al. 2003. Shock 20:493-6.
4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97-100.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.