The BM8.1 monoclonal antibody binds to mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone: BM8.1

Specificity: F4/80 Antigen

Reactivity: Mouse

Format: cFluor^® BYG610¹

Isotype: Rat IgG2b, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES
1. Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640.
2. Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157.
3. Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055.

For research use only. Not intended for use in diagnostic procedures.

¹cFluor^® BYG610 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.