The UCHT1 monoclonal antibody binds to human epsilon chain of the CD3 antigen/T-cell antigen receptor, a 20 to 30-kDa protein complex. This complex contains a CD3γ, a CD3δ, a CD3ζ (CD247), two CD3ε, and a T-cell receptor (α/β or γ/δ) heterodimer ⁽¹⁾. CD3 is found on all mature T cells, NK T cells and thymocytes. It plays a role in recognizing antigen, activating cytotoxic T cell and T helper cell, and signal transduction ⁽²⁾.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: UCHT1

Specificity: CD3

Reactivity: Human, Chimpanzee

Format: cFluor^® B690*

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES
1. Dong, D., et al. 1981. Nature. 573, 546–552
2. Weiss A, et al. 1991. Semin Immunol. (5):313-24

For research use only. Not intended for use in diagnostic procedures.

*cFluor^® B690 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.