PRODUCT DETAILS

Host: Mouse

Isotype: IgG2a, kappa

Clonality: Monoclonal

Clone: OX41

Format: APC

Reactivity: Rt

Application: Flow Cytometry

Tested Dilution: 0.5 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The monoclonal antibody OX41 reacts with rat CD172a, which is also known as signal-regulatory protein alpha (SIRPa). SIRP family members are transmembrane glycoproteins with 3 Ig-like extracellular domains and a cytoplasmic tail containing 2 immunocreceptor tyrosine-based inhibition motifs. CD172a is expressed by monocytes, macrophages, granulocytes, dendritic cells, and neurons. SIRPa association with CD47 inhibits phagocytosis of CD47 presenting cells. Studies suggest that SIRPa ligation prevents phagocytosis by inactivating paxillin and cofilin, proteins involved in cytoskeleton remodeling.

Applications Reported: This OX41 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX41 antibody has been tested by flow cytometric analysis of rat lysed whole blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.