ViaDye Violet Fixable Viability Dye is recommended for dead cell discrimination. It is an amine reactive dye that is impermeable to live cells⁽¹⁾. Dead cells, with their compromised plasma membranes, will preferentially take up the dye. The reactive nature of the dye allows for forming covalent linkages to intracellular proteins. The ViaDye Violet Fixable Viability Dye is compatible with fix/perm protocols.  

PRODUCT DETAILS

Category:            Fixable viability dye

Format:                ViaDye Violet*

Application:        Flow Cytometry

Storage:               -35°C to -10°C and protected from Light. Avoid repeated freeze-thaw cycles

Formulation:       Lyophilized

Data Sheets:       TDS SDS

RECOMMENDED USAGE

The ViaDye Violet Fixable Viability Dye kit is composed of lyophilized ViaDye Violet Fixable Viability Dye and anhydrous DMSO. To reconstitute the dye, bring the kit to room temperature, add 100 µL of DMSO to one vial of the dye, and mix well to ensure that all the dye is in solution. Aliquots of smaller volumes can be made and frozen. It is recommended that the aliquots to not exceed one freeze-thaw cycle.

Note: Cells to be stained with ViaDye Violet Fixable Viability Dye need to be washed and resuspended in protein-free PBS prior to staining.

The suggested use of this reagent is 1 µL of ViaDye Violet Fixable Viability Dye per 1 million cells in a staining volume of 1000 µL.

For staining in 100 µL volumes, the reconstituted dye can be diluted, 1:10, in PBS immediately before use, and 1 µL of the diluted dye added to the cells. It is recommended that users titrate the reagent to obtain the optimal result for their specific applications, as the optimal concentration varies with different cell types.

STAINING PROTOCOL

• Resuspend cells in protein-free PBS after washing.
• Add 1uL of ViaDye to cells and vortex well immediately.
• Incubate for 20 minutes at room temperature, in the dark.
• Wash once with PBS or with FACS Stain buffer, e.g. 0.2% BSA in PBS, pH 7.4.

Cells are ready for downstream staining or applications.

REFERENCES

1. Perfetto SP, et al. 2010 Curr Protoc Cytom. 53:9.34.1

These products are for research use only. Not intended for use in diagnostic procedures.

*ViaDye™ Violet is equivalent to Live-or-Dye™ 375/600 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is coveViolet by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.