PRODUCT DETAILS

Host: Mouse

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: 6H4

Format: Alexa Fluor 647

Reactivity: Ba

Application: Flow Cytometry

Tested Dilution: 0.06 μg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This 6H4 monoclonal antibody recognizes Staphylococcus aureus Cas9 endonuclease.

Applications Reported: This 6H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 6H4 antibody has been tested by intracellular staining followed by flow cytometric analysis of Staphylococcus aureus derived Cas9-GFP transfected 293T cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.