Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.

Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and ELISA.

Applications Tested: Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.