The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.

PRODUCT DETAILS

Clone: VI-01

Specificity: Vimentin

Reactivity: Mammal

Format: Purified

Isotype: Mouse IgM

Application: IHC-P, WB, ICC, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.
Western blotting: Recommended dilution: 1 µg/ml.

REFERENCES

1. Lukás Z, et al. 1989. Histochem J 21:693-701.
2. Lukás Z, et al. 1993. Histochemistry 100:495-502.
3. Bacáková L, et al. 2000. J Biomed Mater Res 49:369-79.
4. Bacáková L, et al. 2000. Biomaterials 21:1173-9.
5. Dráberová E, et al. 1986. Folia Biol (Praha) 32:295-303.

For research use only. Not intended for use in diagnostic procedures.