The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

PRODUCT DETAILS

Clone: ZAP-03

Specificity: ZAP70

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: WB, ICC, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line.
Western blotting: Recommended dilution: 0.5-2 μg/ml.

REFERENCES

1. Chan AC, et al. 1991. Proc Natl Acad Sci U S A 88:9166-70.
2. Ishaq M, et al. 2005. J Biol Chem 280:34152-8.
3. Schneider H, et al. 2008. Eur J Immunol 38:40-7.
4. Hrdinka M, et al. 2011. J Biol Chem 286:19617-29.

For research use only. Not intended for use in diagnostic procedures.