The antibody TU-30 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.

PRODUCT DETAILS

Clone: TU-30

Specificity: Gamma-Tubulin

Reactivity: Human|Mouse, Bovine, Chicken, Pig, Plants, Protozoa, Rat

Format: Purified

Isotype: Mouse IgG1

Application: ICC, WB, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunocytochemistry: Recommended dilution: 1-2 μg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.
Western blotting: Recommended dilution 1-2 μg/ml, reducing conditions.

REFERENCES

1. Wiese C, et al. 2006. J Cell Sci 119:4143-53.
2. Haren L, et al. 2006. J Cell Biol 172:505-15.
3. Sulimenko V, et al. 2006. J Immunol 176:7243-53.
4. Wiese C, et al. 2006. J Cell Sci 119:4143-53.
5. Dráberová L, et al. 1999. Int Immunol 11:1829-39.
6. Haren L, et al. 2006. J Cell Biol 172:505-15.
7. Černohorská M, et al. 2016. Biochim Biophys Acta 1863:1282-97.
8. Katsetos CD, et al. 2006. J Neuropathol Exp Neurol 65:465-77.
9. Dráberová E, et al. 2017. FASEB J 31:1828-1846.
10. Binarová P, et al. 2000. Plant Cell 12:433-42.
11. Libusová L, et al. 2004. Exp Cell Res 295:375-86.
12. Binarová P, et al. 2000. Plant Cell 12:433-42.
13. Klebanovych A, et al. 2019. Cells 8.

For research use only. Not intended for use in diagnostic procedures.