The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

PRODUCT DETAILS

Clone: TU-20

Specificity: Beta III Tubulin

Reactivity: Human|Mouse, Canine, Pig, Rat

Format: Purified

Isotype: Mouse IgG1

Application: FC-IC, WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 1-2 μg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue.
Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

REFERENCES

1. Theodorou E, et al. 2009. Genes Dev 23:575-88.
2. Dráberová E, et al. 2017. FASEB J 31:1828-1846.
3. Jirásek T, et al. 2009. Indian J Med Res 129:89-94.
4. Kukharskyy V, et al. 2004. Exp Cell Res 298:218-28.
5. Jirásek T, et al. 2007. Folia Histochem Cytobiol 45:41-5.
6. Pĕknicová J, et al. 2001. Biol Reprod 65:672-9.
7. Dráberová E, et al. 1998. Histochem Cell Biol 109:231-9.

For research use only. Not intended for use in diagnostic procedures.