The mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.

PRODUCT DETAILS

Clone: EM-22

Specificity: Beta-Catenin

Reactivity: Human|Mouse, Hamster

Format: Purified

Isotype: Mouse IgG2a

Application: IP, WB, ICC, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line.
Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

REFERENCES

1. Nowak M, et al. 2007. Folia Histochem Cytobiol 45:233-8.
2. Jung IM, et al. 2007. J Korean Med Sci 22:855-61.
3. Valenta T, et al. 2003. Nucleic Acids Res 31:2369-80.

For research use only. Not intended for use in diagnostic procedures.