The rat monoclonal antibody YOL1/34 recognizes an epitope of alpha-tubulin localized between amino acids 414-422. It has higher affinity for fixed microtubules than for native ones.

PRODUCT DETAILS

Clone: YOL1/34

Specificity: Alpha Tubulin

Reactivity: Human|Mouse, Avian, Mammal, Rat, Yeast

Format: Purified

Isotype: Rat IgG2a

Application: WB, IHC-P, ICC, ELISA, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunocytochemistry: Recommended dilution 4-8 μg/ml.
Western blotting: Recommended dilution 1-2 μg/ml; reducing conditions.
Flow cytometry: Recommended dilution: 8-12 μg/ml; intracellular staining.

REFERENCES

1. Badin-Larçon AC, et al. 2004. Proc Natl Acad Sci U S A 101:5577-82.
2. Harris GS, et al. 1989. Mol Cell Biol 9:2042-9.
3. Larcher JC, et al. 1996. J Biol Chem 271:22117-24.
4. Breitling F, et al. 1986. J Mol Biol 189:367-70.

For research use only. Not intended for use in diagnostic procedures.