The antibody MEM-G/1 reacts with an extracellular epitope of denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.

PRODUCT DETAILS

Clone: MEM-G/1

Specificity: MHC Class I (HLA-G)

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: WB, IHC-P

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Recommended dilution: 1:60 to 1:100, incubation: 1 h at RT; positive tissue: human placenta - extravillous cytotrophoblast, heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven).
Western blotting: Recommended dilution 1-2 μg/ml.

REFERENCES

1. Créput C, et al. 2003. J Hepatol 39:587-94.
2. Menier C, et al. 2003. Hum Immunol 64:315-26.
3. Lin A, et al. 2018. Front Immunol 9:2164.
4. Hunt JS, et al. 2006. Reprod Biol Endocrinol 4 Suppl 1:S10.
5. Xu HH, et al. 2020. Front Immunol 11:1349.
6. Castelli EC, et al. 2021. Sci Rep 11:23070.
7. Curigliano G, et al. 2013. Clin Cancer Res 19:5564-71.
8. Hurks HM, et al. 2001. Invest Ophthalmol Vis Sci 42:3081-4.
9. Gonen-Gross T, et al. 2005. J Immunol 175:4866-74.
10. Shih IM, et al. 2004. Am J Surg Pathol 28:1177-83.
11. Boyson JE, et al. 2002. Proc Natl Acad Sci U S A 99:16180-5.
12. Frumento G, et al. 2000. Tissue Antigens 56:30-7.
13. Fournel S, et al. 2000. Tissue Antigens 55:510-8.
14. Zhang X, et al. 2020. Front Immunol 11:565759.
15. Swets M, et al. 2018. Clin Immunol 194:80-86.
16. Menier C, et al. 2003. Hum Immunol 64:315-26.
17. Lodererová A, et al. 2003. Folia Microbiol (Praha) 48:239-42.

For research use only. Not intended for use in diagnostic procedures.