The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

PRODUCT DETAILS

Clone: 5A6G7

Specificity: MHC Class I (HLA-G)

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: WB, IHC-P, ICC, ELISA, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml; positive tissue: placenta.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: placenta.
ELISA: Positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100).
Western blotting: Positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE.

REFERENCES

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For research use only. Not intended for use in diagnostic procedures.