The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.

PRODUCT DETAILS

Clone: A53-B/A2

Specificity: Cytokeratin 19

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2a

Application: FC-IC, IHC-P, IP, WB, ICC, ELISA

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml, positive control: MCF-7, BA-17, HT-29 cells.

REFERENCES

1. Karsten U, et al. 1985. Eur J Cancer Clin Oncol 21:733-40.
2. Kasper M, et al. 1988. Histochemistry 89:369-77.
3. Bártek J, et al. 1986. Histochem J 18:565-75.
4. Quardokus EM, et al. 2023. Nat Methods 20:1174-1178.

For research use only. Not intended for use in diagnostic procedures.