The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

PRODUCT DETAILS

Clone: MEM-43/5

Specificity: CD59

Reactivity: Human, Mouse

Format: Purified

Isotype: Mouse IgG2b

Application: WB, FC, IP, IHC-P

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: blood.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.

REFERENCES

1. Baalasubramanian S, et al. 2004. J Immunol 173:3684-92.
2. Menu E, et al. 1994. J Immunol 153:2444-56.
3. Rooney IA, et al. 1991. Clin Exp Immunol 83:251-6.
4. Meri S, et al. 1990. Immunology 71:1-9.
5. Stulnig TM, et al. 1997. J Biol Chem 272:19242-7.
6. Drbal K, et al. 2007. Int Immunol 19:675-84.
7. Bodian DL, et al. 1997. J Exp Med 185:507-16.
8. Ohradanova-Repic A, et al. 2018. Nanomedicine 14:123-130.

For research use only. Not intended for use in diagnostic procedures.