The antibody MEM-32 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes).

PRODUCT DETAILS

Clone: MEM-32

Specificity: CD5

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: FC, IP, WB, IHC-P, ELISA

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

ELISA: The antibody MEM-32 can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody CRIS1.
Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/ml; positive tissue: spleen.
Flow cytometry: Recommended dilution: 2 μg/ml.
Western blotting: Laurylmaltoside lysing buffer; non-reducing conditions; recommended dilution: 1-2 μg/ml.

REFERENCES

1. Freedman AS, et al. 1989. Eur J Immunol 19:849-55.
2. Raman C. 2002. Immunol Res 26:255-63.
3. Hrdinka M, et al. 2011. J Biol Chem 286:19617-29.
4. Horejsí V, et al. 1986. Folia Biol (Praha) 32:12-25.

For research use only. Not intended for use in diagnostic procedures.