The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.

PRODUCT DETAILS

Clone: MEM-263

Specificity: CD44

Reactivity: Human, Canine, Pig

Format: Purified

Isotype: Mouse IgG1

Application: FC, IP, WB, IHC-P

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 4 μg/ml.
Western blotting: Recommended dilution: 2 μg/ml, 60 min on vertical incubator; positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6% separating gel.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: uterus, myometrium.

REFERENCES

1. Vigetti D, et al. 2008. J Biol Chem 283:4448-58.
2. Hollingsworth JW, et al. 2007. Am J Respir Cell Mol Biol 37:248-53.
3. Liu J, et al. 2007. Cell Mol Immunol 4:59-63.
4. Subramaniam V, et al. 2007. Exp Mol Pathol 83:341-6.
5. Subramaniam V, et al. 2007. Exp Mol Pathol 83:207-15.

For research use only. Not intended for use in diagnostic procedures.