The mouse monoclonal antibody clone SK7 binds to human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR) and the CD3 zeta chain to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex recognizes antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines, or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The SK7 antibody can be used as a phenotypic marker for human T cells. An alternative Anti-Human CD3 clone, OKT3, can block the binding of clone SK7.

PRODUCT DETAILS

Clone: SK7

Specificity: CD3

Reactivity: Human, Chimpanzee

Format: Purified

Isotype: Mouse IgG1, k

Application: FC, WB, ICC, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 0.5-3 μg/ml. Extracellular and intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.

For research use only. Not intended for use in diagnostic procedures.