The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.

PRODUCT DETAILS

Clone: EM-20

Specificity: CD230

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2a

Application: WB

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 0.5 μg/ml, non-reducing conditions are essential.

REFERENCES

1. Li C, et al. 2009. J Clin Invest 119:2725-36.
2. Ermonval M, et al. 2009. PLoS One 4:e6497.
3. Yang Y, et al. 2009. BMB Rep 42:331-7.
4. Segarra C, et al. 2009. PLoS One 4:e5796.
5. Wang X, et al. 2009. BMB Rep 42:444-9.
6. Dvorakova E, et al. 2013. BMC Neurol 13:126.

For research use only. Not intended for use in diagnostic procedures.