The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

PRODUCT DETAILS

Clone: MEM-229

Specificity: CD105

Reactivity: Human, Pig

Format: Purified

Isotype: Mouse IgG2a

Application: FC, WB, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 2-6 μg/ml; positive control: Kg1 human acute myelogenous leukemia cell line.
Immunohistochemistry (frozen sections): Recommended dilution: 1:200, acetone fixation.
Western blotting: Non-reducing conditions.

REFERENCES

1. Zhu Y, et al. 2003. Stroke 34:2483-8.
2. Li C, et al. 2003. J Cell Sci 116:2677-85.
3. Guo B, et al. 2004. Anticancer Res 24:1337-45.
4. Warrington K, et al. 2005. Anticancer Res 25:1851-64.
5. Piao M, et al. 2006. J Atheroscler Thromb 13:82-9.
6. Plánka L, et al. 2009. Physiol Res 58:885-893.

For research use only. Not intended for use in diagnostic procedures.