Phagocytosis is a complex process for the ingestion and elimination of pathogens. It is also important for the elimination of apoptotic cells, hence fundamental for tissue homeostasis. Phagocytosis consists of recognition and ingestion of particles larger than 0.5 µm into a plasma membrane-derived vesicle, known as phagosome. Professional phagocytes include monocytes, macrophages, neutrophils, dendritic cells, osteoclasts, and eosinophils. These cells are in charge of eliminating microorganisms and presenting them to cells of the adaptive immune system. In addition, fibroblasts, epithelial cells, and endothelial cells can also perform phagocytosis. These nonprofessional phagocytes cannot ingest microorganisms but are important in eliminating apoptotic bodies. Once internalized, the phagosome fuse with lysosomes to form secondary phagolysosomes for digestion, resulting in progressive decrease of pH. Streamline your phagocytosis research with BioLegend’s Phagocytosis Detection Kit Red. This ready-to-use fluorimetric assay kit is designed for analysis of phagocytic activity in live cells. This kit provides a simple, sensitive method to monitor cellular uptake using pH-activated Phagocytosis Detection Beads. These latex beads are conjugated to a red dye which is non-fluorescent at neutral pH but dramatically increases fluorescence upon internalization into acidic phagosomes. This kit also includes Viability Dye Green enabling simultaneous measurement of cellular uptake and cell viability in a single assay. This dual-color approach provides critical context by distinguishing between reduced phagocytosis due to cellular dysfunction versus specific inhibition of the phagocytic process.

PRODUCT DETAILS

Clone: None

Antibody Type: None

Reactivity: Human, Mouse

Format: None

Host: None

Isotype: None

Applications: Live cell imaging - Quality tested FC - Verified

Regulatory Status: RUO

Data Sheets: TDS | SDS

Note to Purchaser: This product is manufactured and supplied by BioLegend, Inc. under an agreement between BioLegend, Inc. and Cytek Biosciences, Inc. Information related to this product is provided by BioLegend, Inc.