PerCP-Cyanine5.5 Anti-Mouse CD8a (53-6.7)
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説明
The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.
The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
Recent Publications:
C Khouili S, Cook ECL, Hernández-García E, Martínez-López M, Conde-Garrosa R, Iborra S. SHP-1 Regulates Antigen Cross-Presentation and Is Exploited by Leishmania to Evade Immunity. Cell Rep. 2020 Dec 1;33(9):108468. doi: 10.1016/j.celrep.2020.108468. PMID: 33264612.
Hiroshi Kobayashi and Keiyo Takubo. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protocols. Published 2 August 2020. https://doi.org/10.1016/j.xpro.2020.100078.
Kobayashi H, Morikawa T, Okinaga A, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28(1):145-158.e9. doi:10.1016/j.celrep.2019.06.008.
Finkin S, Yuan D, Stein I, Taniguchi K, Weber A, Unger K, Browning JL, Goossens N, Nakagawa S, Gunasekara G, Schwartz ME et al. 2015. Nat Immunol. doi: 10.1038/ni.3290. (Flow Cytometry)
Name | PerCP-Cyanine5.5 Anti-Mouse CD8a (53-6.7) |
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Cat. No. | 65-0081 |
Alternative Names | CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2 |
Gene ID | 12525 |
Clone | 53-6.7 |
Isotype | Rat IgG2a, κ |
Reactivity | Mouse |
Format | PerCP-Cyanine5.5 |
Application | Flow Cytometry |