The mouse monoclonal antibody clone JOVI.1 binds to a constant region of the T cell receptor beta chain, also known as T cell receptor beta constant 1 (TRBC1). It is expressed on a subset of TCRαβ T cells on the beta chain of the T Cell receptor. The immunogen used to generate the hybridoma were cells from transgenic animals expressing the human Vβ3-Cβ1 TCR chain that was cloned from the HA1.7 human T cell line.

PRODUCT DETAILS

Clone: JOVI.1

Specificity: TCR Cβ1

Reactivity: Human

Format: PerCP-Cyanine5.5

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Minden, M D and T W Mak. 1986. Blood. 68:327
2. Davis, M M et al. 1997. Ciba Found Symp. 204:94
3. Maciocia, Paul M et al. 2017. Nat Med. 23:1416

For research use only. Not intended for use in diagnostic procedures.

PerCP-Cyanine5.5 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.