The antibody MEM-31 recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells; negative in Western blotting application.

PRODUCT DETAILS

Clone: MEM-31

Specificity: CD8

Reactivity: Human

Format: PE-Cyanine7

Isotype: Mouse IgG2a

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Devine L, et al. 2006. J Immunol 177:3930-8.
2. Pang DJ, et al. 2007. J Biol Chem 282:13884-94.
3. van den Berg HA, et al. 2007. J Theor Biol 249:395-408.
4. Kanderova V, et al. 2016. Mol Cell Proteomics 15:1246-61.
5. Linnebacher M, et al. 2010. J Biomed Biotechnol 2010:841451.
6. Estefanía E, et al. 2007. J Immunol 178:4402-10.
7. Drbal K, et al. 2007. Int Immunol 19:675-84.
8. Brdicková N, et al. 2003. J Exp Med 198:1453-62.
9. Horejsí V, et al. 1988. Folia Biol (Praha) 34:23-34.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.