![Human peripheral blood lymphocytes were stained with 5 uL (0.25 ug) PE-Cy7 Anti-Human CD27 (60-0279) (solid line) or 0.25 ug PE-Cy7 Mouse IgG1 isotype control (dashed line).](http://cytekbio.com/cdn/shop/products/60-0279_large.png?v=1675105543)
PE-Cyanine7 Anti-Human CD27 (O323)
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説明
The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of ≥ 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.
The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.
Name | PE-Cyanine7 Anti-Human CD27 (O323) |
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Cat. No. | 60-0279 |
Alternative Names | TNFRSF7, S152, T14 |
Gene ID | 939 |
Clone | O323 |
Isotype | Mouse IgG1, kappa |
Reactivity | Human |
Cross Reactivity | Baboon, Cynomolgus, Rhesus |
Format | PE-Cyanine7 |
Application | Flow Cytometry |