The mouse monoclonal antibody clone LT21 binds to an extracellular epitope of human CD21, a 145 kDa type I transmembrane glycoprotein also known as complement receptor 2 (CR2), C3d receptor (C3dR), and EBV receptor. CD21 is expressed on mature B lymphocytes, follicular dendritic cells, a subset of T lymphocytes, and some epithelial cells, but is absent on immature B cells. CD21 functions as a receptor for complement fragments C3d, C3dg, and iC3b, which remain covalently attached to complement-activating surfaces and antigens. On the B cell surface, CD21 associates with CD19, CD81, and Leu-13 to form a large co-receptor signaling complex that amplifies B cell receptor activation in response to complement-opsonized antigens. CD21 also serves as the obligate receptor for Epstein-Barr virus (EBV), mediating viral binding and entry into B lymphocytes via interaction with the viral glycoprotein gp350/220. Additional roles for CD21 include uptake and retention of immune complexes, survival of memory B cells, and maintenance of the humoral response to T-dependent antigens. Clone LT21 has been validated for flow cytometric analysis of human peripheral blood leukocytes and has been reported to cross-react with canine and porcine CD21.

PRODUCT DETAILS

Clone: LT21

Specificity: CD21

Reactivity: Human, Dog, Pig

Format: PE-Cyanine7

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Weis, J.J. et al. 1984. Proc. Natl. Acad. Sci. USA. 81: 881–885.
2. Matsumoto, A.K. et al. 1993. J. Exp. Med. 178: 1407–1417.
3. Fearon, D.T. and Carter, R.H. 1995. Annu. Rev. Immunol. 13: 127–149.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.