The mouse monoclonal antibody clone 4A11 binds to the Fab fragment of human IgG. IgG is a membrane BCR that is produced when activated naïve B cells undergo class switch recombination of the Ig heavy chain constant region. This class-switching results in IgM and IgD being replaced by IgG as the activated B cell develops into memory and antibody-secreting cells. On memory B cells, when IgG binds to its target antigen, it initiates signaling and activation that rapidly leads to proliferation and differentiation into antibody-secreting cells.

PRODUCT DETAILS

Clone: 4A11

Specificity: IgG

Reactivity: Human

Format: PE

Isotype: Mouse IgG2b

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

For research use only. Not intended for use in diagnostic procedures.