The antibody HI40a recognizes an extracellular epitope of CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.

PRODUCT DETAILS

Clone: HI40a

Specificity: CD40

Reactivity: Human

Format: PE

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Oxenius A, et al. 1996. J Exp Med 183:2209-18.
2. Grewal IS, et al. 1996. Immunol Rev 153:85-106.
3. Pearson LL, et al. 2001. Int Immunol 13:273-83.
4. Wu W, et al. 2008. Toxicol Appl Pharmacol 228:135-43.
5. Súkeníková L, et al. 2017. Immunol Lett 189:82-89.

For research use only. Not intended for use in diagnostic procedures.