The mouse monoclonal antibody clone EH12.2H7 binds to human CD279 (PD-1), an inhibitory checkpoint receptor expressed on activated T cells, B cells, NK cells, and subsets of myeloid cells. CD279 plays a central role in immune exhaustion, tolerance, and suppression during chronic antigen exposure and malignancy. Clone EH12.2H7 binds an extracellular epitope and is suited for flow cytometric profiling of T-cell exhaustion, immune checkpoint expression, and tumor-infiltrating lymphocyte characterization

PRODUCT DETAILS

Clone: EH12.2H7

Specificity: CD279 (PD-1)

Reactivity: Human, African Green Monkey, Baboon, Chimpanzee, Common Marmoset, Cynomolgus Monkey, Rhesus Macaque, Squirrel Monkey, Non-Human Primate

Format: PE

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Dong H, et al. 2002. Nat Med. 8:793–800.
2. Wherry EJ. 2011. Nat Immunol. 12:492–499.
3. Gros A, et al. 2014. J Clin Invest. 124:2246–2259.

For research use only. Not intended for use in diagnostic procedures.