The antibody MEM-238 recognizes an extracellular epitope between amino acids 192-697 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

PRODUCT DETAILS

Clone: MEM-238

Specificity: CD222

Reactivity: Human, Non-Human Primate

Format: PE

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Wood RJ, et al. 2008. J Biol Chem 283:4165-76.
2. Gasanov U, et al. 2006. Infect Immun 74:566-77.
3. Machacek C, et al. 2016. J Immunol 197:2229-38.
4. Leksa V, et al. 2002. J Biol Chem 277:40575-82.
5. Schatzlmaier P, et al. 2015. Sci Signal 8:rs11.
6. Leksa V, et al. 2012. J Biol Chem 287:22450-62.
7. Schiller HB, et al. 2009. Mol Biol Cell 20:745-56.
8. Leksa V, et al. 2011. Circ Res 108:676-85.

For research use only. Not intended for use in diagnostic procedures.