The mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes an extracellular epitope of CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system.

PRODUCT DETAILS

Clone: HTF-1

Specificity: CD142

Reactivity: Human

Format: PE

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Bucciarelli P, et al. 2012. Thromb Res 129:591-7.
2. Patil R, et al. 2013. PLoS One 8:e81407.
3. Aass HC, et al. 2011. Cytometry A 79:990-9.
4. Carson SD, et al. 1987. Blood 70:490-3.
5. Virtanen S, et al. 2021. Breast Cancer Res 23:66.

For research use only. Not intended for use in diagnostic procedures.