The mouse monoclonal antibody clone 3B2/TA8 binds to human CD99, also known as E2 antigen and MIC2. CD99 is an approximately 32 kDa sialoglycoprotein and is expressed by all leukocytes. CD99 is involved in T cell adhesion and activation. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas and is absent on some B cell lines, fetal B cells, eosinophils, and granulocytes. CD99 is involved in spontaneous erythrocyte rosette formation and may be involved in other T cell and hematopoietic cell adhesion pathways.

PRODUCT DETAILS

Clone: 3B2/TA8

Specificity: CD99

Reactivity: Human

Format: Pacific Blue™

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Waclavicek, M., et al. 1998. J. Immunol 161:4671-8.

For research use only. Not intended for use in diagnostic procedures.