The Muse^® Oxidative Stress kit allows for the quantitative measurements of Reactive Oxygen Species (ROS), namely superoxide radicals in cells undergoing oxidative stress. The assay provides the relative percentage of cells that are ROS negative and positive in both adherent cells and cells in suspension on the Guava^® Muse® cell analyzer. Minimal sample preparation is required in this no-wash, mix-and-read assay, making it easy to obtain accurate and precise results. The Muse Oxidative Stress kit simultaneously determines the count and percentage of cells undergoing oxidative stress based on the intracellular detection of superoxide radicals. The kit utilizes the Muse Oxidative Stress reagent for the detection of ROS in cells. The Muse Oxidative Stress reagent is based on dihydroethidium, a well-characterized reagent that has been extensively used to detect reactive oxidative species in cellular populations.
Two populations of cells can be distinguished in the assay:

• ROS(-): Live cells
• ROS(+): Cells exhibiting ROS

The Muse Oxidative Stress kit is optimized for use with Muse system, which makes sophisticated, fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse cell analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.

PRODUCT DETAILS

Application:        Flow Cytometry

Storage:              See Documentation

Data Sheets:       QRC  SDS

EXAMPLE DATA

Recommended Staining Protocol