The Muse^® Human B Cell kit allows for determination of the B cell count, total lymphocyte count, and B cell percent of lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. Characterization of B cell counts and percentages enables a better understanding of the humoral immune response, insight into the mechanisms of autoimmune diseases, further research into factors that influence T cell responses, and improved vaccine development.

The Muse Human B Cell kit detects and identifies lymphocytes and CD19+ B lymphocytes in both whole blood and PBMCs using a simplified, no-wash assay. Because CD19 is considered to be a characteristic B cell marker, it is commonly used in the routine immunophenotyping of B cells. Monoclonal antibodies cluster as CD19 detects all peripheral blood B cells.1 The simplified identification aids in understanding the role of B lymphocytes in development, response, and mechanism of disease.

The Muse Human B Cell kit is optimized for use with the Guava^® Muse^® cell analyzer, which makes sophisticated, fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse cell analyzer, intuitive software provides detailed or summary analysis of your cell sample in a few short steps. The software readout on the Muse cell analyzer provides:

• Count, percentage, and concentration of B cells
• Count and concentration of lymphocytes

PRODUCT DETAILS

Application:        Flow Cytometry

Storage:              See Documentation

Data Sheets:       QRC   SDS

EXAMPLE DATA

Recommended Staining Protocol