The antioxidant reduced glutathione (GSH) plays an essential role in preserving a reduced intracellular environment, and protects cellular macromolecules like DNA, proteins and lipids against oxidizing agents. Reactive oxygen species can oxidize cellular GSH, leading to the loss of intracellular redox homeostasis and activation of the apoptotic signaling cascade.The Intracellular Glutathione Assay Kit is designed to measure GSH in live cells by flow cytometry and live-cell imaging. This fluorometric assay is based on the detection of GSH in cells using the GSH Probe Green, which is non-fluorescent in its native state but becomes strongly fluorescent upon reacting with glutathione. Fluorescence intensity decreases in cells with low GSH levels, as well as in cells undergoing apoptosis.This kit provides enough reagents for 100 tests (100 mL total working solution for up to 1x108cells). A single test is defined as 1 mL of working solution for up to 1x106cells.

PRODUCT DETAILS

Clone: None

Antibody Type: None

Reactivity: Human

Format: None

Host: None

Isotype: None

Applications: Live cell imaging - Quality tested FC - Verified

Regulatory Status: RUO

Data Sheets: TDS | SDS

Note to Purchaser: This product is manufactured and supplied by BioLegend, Inc. under an agreement between BioLegend, Inc. and Cytek Biosciences, Inc. Information related to this product is provided by BioLegend, Inc.