The Guava^® DNA Damage Histone H2A.X Dual Detection kit is part of a series of flow cytometry products that include a pair of antibodies which bind to the same protein; one antibody detects total protein expression and the other detects the phosphorylated form of the same target. Two-parameter analysis allows for the specific detection of phosphorylation and eliminates false positives, enhancing the signal-to-noise ratio.

The kit includes two directly conjugated antibodies-a phospho-specific anti-phospho-Histone H2A.X (Ser139)-PerCP and an anti-Histone H2A.X-FITC conjugated antibody-to measure total levels of Histone H2A.X expression. This two-color kit is designed to detect the extent of Histone H2A.X pathway activation by measuring H2A.X phosphorylation relative to the total H2A.X expression in any given cell population. By doing this, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of H2A.X activation after treatment. Simultaneous measurement of both total and phospho-Histone H2A.X confirms target specificity of the phosphorylation event.

A comprehensive understanding of both Histone H2A.X activity and the consequence of its activation can provide researchers with useful information in characterizing the DNA damage response. The Histone H2A.X DNA Damage Dual Detection kit is designed to allow the researcher to monitor and accurately measure phospho-specific Histone H2A.X activation in a population of cells.

PRODUCT DETAILS

Application:        Flow Cytometry

Storage:              See Documentation

EXAMPLE DATA

Recommended Staining Protocol

For Research Use Only. Not intended for use in diagnostic procedures.