PRODUCT DETAILS

Host: Mouse

Isotype: IgG2a, kappa

Clonality: Monoclonal

Clone: N4TL33

Format: eFluor 450

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 5 µL (0.5 µg)/test

Concentration: 5 μL/Test

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Applications Reported: This N4TL33 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This N4TL33 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor™ 450 is an alternative to Pacific Blue™. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.