TDS | SDS

Tonbo Biosciences Flow Cytometry Perm Buffer (10X) is provided as a concentrate which, when diluted with distilled water to a 1X solution, provides best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps. The Flow Cytometry Perm Buffer is formulated for optimal resolution and low background when used in fluorophore-labeled antibody staining protocols, followed by flow cytometric analysis.

Download the Intracellular Cytokine Staining Protocol.

Recent Publications:

Swain N, Tripathy A, Padhan P, Raghav SK, Gupta B. Toll-like receptor-7 activation in CD8+ T cells modulates inflammatory mediators in patients with rheumatoid arthritis. Rheumatol Int. 2022 Feb 10. doi: 10.1007/s00296-021-05050-8. Epub ahead of print. PMID: 35142867.  I

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Jesser EA, Brady NJ, Huggins DN, Witschen PM, O'Connor CH, Schwertfeger KL. STAT5 is activated in macrophages by breast cancer cell-derived factors and regulates macrophage function in the tumor microenvironment. Breast Cancer Res. 2021 Nov 7;23(1):104. doi: 10.1186/s13058-021-01481-0. PMID: 34743736; PMCID: PMC8573892.

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Xu K, Li MO. Delivery of membrane impermeable molecules to primary mouse T lymphocytes. STAR Protoc. 2021 Aug 17;2(3):100757. doi: 10.1016/j.xpro.2021.100757. PMID: 34458872; PMCID: PMC8377589.

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Poudel B, Yorek MS, Mazgaeen L, Brown SA, Kanneganti TD, Gurung P. Acute IL-4 Governs Pathogenic T Cell Responses during Leishmania major Infection. Immunohorizons. 2020 Sep 18;4(9):546-560. doi: 10.4049/immunohorizons.2000076. PMID: 32948646.

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Kotov JA, Kotov DI, Linehan JL, Bardwell VJ, Gearhart MD, Jenkins MK. BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors. J Exp Med. 2019;216(6):1450-1464. doi:10.1084/jem.20182376.

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Kevin C. Osum, Adam L. Burrack, Tijana Martinov, Nathanael L. Sahli, Jason S. Mitchell, Christopher G. Tucker, Kristen E. Pauken, Klearchos Papas, Balamurugan Appakalai, Justin A. Spanier, & Brian T. Fife. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9

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Lesteberg K, Orange J and Makedonas G. 2017. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res. doi: 10.1007/s12026-017-8945-8.

Colombo E, Tentorio P, Musio S, Rajewsky K, Pedotti R, Casola S, and Farina C. 2014. Clin. Exp. Immunol. 176:58-65. (Flow Cytometry)