The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

PRODUCT DETAILS

Clone: M5/114

Specificity: MHC Class II (I-A/I-E)

Reactivity: Mouse

Format: FITC

Isotype: Rat IgG2b

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 2-5 μg/ml. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Clausen BE, et al. 1998. Immunity 8:143-55.
2. Zang W, et al. 2005. J Am Soc Nephrol 16:3661-8.
3. Kuwano Y, et al. 2007. Int Immunol 19:977-92.
4. Beers C, et al. 2005. J Immunol 174:1205-12.
5. Kleijmeer M, et al. 2001. J Cell Biol 155:53-63.
6. De Souza Leao S, et al. 1995. J Cell Sci 108 ( Pt 10):3219-31.
7. Viville S, et al. 1993. Cell 72:635-48.
8. Bhattacharya A, et al. 1981. J Immunol 127:2488-95.

For research use only. Not intended for use in diagnostic procedures.