The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

PRODUCT DETAILS

Clone: APA1/1

Specificity: CD3 Epsilon (Activation Epitope)

Reactivity: Human|Mouse

Format: FITC

Isotype: Mouse IgG1

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 1-5 μg/ml. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Risueño RM, et al. 2006. Proc Natl Acad Sci U S A 103:9625-30.
2. Alarcón B, et al. 1991. EMBO J 10:903-12.
3. Gil D, et al. 2005. J Exp Med 201:517-22.
4. Risueño RM, et al. 2005. Blood 106:601-8.
5. Delgado P, et al. 2000. Nature 406:426-30.
6. Tailor P, et al. 2008. J Immunol 181:243-55.
7. Rieux-Laucat F, et al. 2006. N Engl J Med 354:1913-21.
8. Torres PS, et al. 2003. J Immunol 170:5947-55.
9. Wong AP, et al. 2007. Am J Physiol Lung Cell Mol Physiol 293:L740-52.

For research use only. Not intended for use in diagnostic procedures.