TDS | SDS

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.

Recent Publications:

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Raihan MO, McGregor BA, Velaris NA, Brishti A, Hur J, Porter JE. Method of Microglial DNA-RNA Purification from a Single Brain of an Adult Mouse. Methods Protoc. 2021 Dec 2;4(4):86. doi: 10.3390/mps4040086. PMID: 34940397; PMCID: PMC8704779.

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Baba T, Yoshida T, Tanabe Y, et al. Cytoplasmic DNA accumulation preferentially triggers cell death of myeloid leukemia cells by interacting with intracellular DNA sensing pathway. Cell Death Dis. 2021 Mar 26;12(4):322. doi: 10.1038/s41419-021-03587-x. PMID: 33771977.  

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Li Y, Han Y, Su R, Liu Y, Chong G, Xu D, He R, Liu Y, Dong H, Shi D, Li Y. Photosensitizer-Laden Neutrophils Are Controlled Remotely for Cancer Immunotherapy. Cell Rep. 2020 Dec 15;33(11):108499. doi: 10.1016/j.celrep.2020.108499. PMID: 33326787. 

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Bescucci DM, Clarke ST, Brown CLJ, et al. The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection. Gut Pathog. 2020 Nov 13;12(1):53. doi: 10.1186/s13099-020-00386-1. PMID: 33292444; PMCID: PMC7666523.

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Komura N, Mabuchi S, Shimura K, et al. The role of myeloid-derived suppressor cells in increasing cancer stem-like cells and promoting PD-L1 expression in epithelial ovarian cancer. 2020 Jun 19. Cancer Immunol Immunother. 2020;10.1007/s00262-020-02628-2. doi:10.1007/s00262-020-02628-2.

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Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.

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Palacios M, Miner TA, Frederick DR, et al. Identification of Two Regulators of Virulence That Are Conserved in Klebsiella pneumoniae Classical and Hypervirulent Strains. mBio. 2018;9(4):e01443-18. Published 2018 Aug 7. doi:10.1128/mBio.01443-18.

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Kawano M, Mabuchi S, Matsumoto Y, Sasano T et al. 2015. Sci Rep. 5:18217 doi:10.1038/srep18217. (Flow Cytometry)