The antibody MEM-85 reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.

PRODUCT DETAILS

Clone: MEM-85

Specificity: CD44

Reactivity: Human

Format: FITC

Isotype: Mouse IgG2b

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Vigetti D, et al. 2008. J Biol Chem 283:4448-58.
2. Hollingsworth JW, et al. 2007. Am J Respir Cell Mol Biol 37:248-53.
3. Liu J, et al. 2007. Cell Mol Immunol 4:59-63.
4. Subramaniam V, et al. 2007. Exp Mol Pathol 83:341-6.
5. Subramaniam V, et al. 2007. Exp Mol Pathol 83:207-15.
6. Kanderova V, et al. 2016. Mol Cell Proteomics 15:1246-61.
7. Schmidt D, et al. 2007. Circulation 116:I64-70.
8. Ilangumaran S, et al. 1998. Blood 91:3901-8.
9. Bazil V, et al. 1994. J Immunol 152:1314-22.
10. Campioni D, et al. 2006. Haematologica 91:364-8.

For research use only. Not intended for use in diagnostic procedures.