The antibody LT20 reacts with an extracellular epitope of CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

PRODUCT DETAILS

Clone: LT20

Specificity: CD20

Reactivity: Human

Format: FITC

Isotype: Mouse IgG2a

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Li C, et al. 2007. Biochem J 406:333-41.
2. Glennie MJ, et al. 2007. Mol Immunol 44:3823-37.
3. Cragg MS, et al. 2005. Curr Dir Autoimmun 8:140-74.
4. Petrie RJ, et al. 2002. J Immunol 169:2886-91.
5. Hultin LE, et al. 1993. Cytometry 14:196-204.
6. Všianská P, et al. 2015. Clin Lymphoma Myeloma Leuk 15:e61-71.
7. Filatov AV, et al. 2007. J Immunol Methods 319:21-33.
8. Kanderova V, et al. 2016. Mol Cell Proteomics 15:1246-61.
9. Polyak MJ, et al. 2002. Blood 99:3256-62.
10. Chan HT, et al. 2003. Cancer Res 63:5480-9.
11. Teeling JL, et al. 2006. J Immunol 177:362-71.

For research use only. Not intended for use in diagnostic procedures.