The mouse monoclonal antibody clone 3G8 binds to human IgG receptor III (FcγRIII) that are in two forms: CD16a (FcγRIIIA) and CD16b (FcγRIIIB). With 95% sequence similarity, they are a conventional 50-65 kDa polypeptide-anchored transmembrane protein and a 48 kDa GPI-anchored protein, respectively. CD16a is expressed on NK cells and macrophages, while CD16b is expressed on neutrophils. CD16a also plays a crucial role in antibody-dependent cellular cytotoxicity (ADCC) by NK cells.

PRODUCT DETAILS

Clone: 3G8

Specificity: CD16

Reactivity: Human, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cynomolgus Monkey, Pigtail Monkey, Rhesus Macaque, Sooty Mangabey, Squirrel Monkey

Format: FITC

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.

For research use only. Not intended for use in diagnostic procedures.