Cytek® cFluor® MDSC Kit
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説明
Myeloid-derived suppressor cells (MDSCs) are neutrophils and monocytes that possess potent immunosuppressive activity. The Cytek® cFluor® MDSC Kit has been designed and optimized to identify and analyze MDSCs. The panel includes markers to identify MDSCs and MDSC subsets such as, monocytic MDSC (M-MDSC) and granuclocytic/polymorphonuclear MDSC (PMN-MDSC). Newly identified MDSC markers such as, CD84, LOX-1, and CD181, help to delineate the MDSC subsets and also serve as indictors of cell status. MDSCs have been shown to play an important role in regulating immune responses in cancer and other pathological conditions, including chronic infection, sepsis, and autoimmunity.
PRODUCT DETAILS
Tested Dilution: 5 µL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)
The panel contains the following single vial reagents.
Table 1: Composition of the Cytek® cFluor® MDSC Kit.
DATA
Figure 1. Cell gating hierarchy for identifying MDSCs using the Cytek® cFluor® MDSC Kit. Data was generated using whole blood collected in EDTA from a healthy donor. Sequential gates are set to select single cells and remove debris. Total white blood cells are identified as CD45+ single cells. Additional gates are followed to exclude eosinophils, basophils, potentially contaminated platelets, and CD66+CD16++ classical neutrophils. The remaining cells are used to gate on lymphoid lineage negative and HLA-DRlow or negative cells. From the CD33+CD11b+ gate myeloid cells that contain the MDSC population are further classified into CD14+ M-MDSCs and CD15+ PMN-MDSCs. An additional gate on the CCR3– region further eliminates eosinophil contamination if present. The level of expression of the additional markers CD84, CD181, and LOX-1 are assessed on MDSC subsets.
RECOMMENDED USAGE
Whole blood collected in K2EDTA, Heparin, ACD, and Cyto-Chex® BCT blood tubes have been tested to validate the performance of this kit. PBMCs and tissue infiltrating leukocytes have also been tested and validated. Please refer to the section below to download this kit's staining protocols, data acquisition protocols, experiment templates, and fluorochrome list.
Use appropriate personal protective equipment per product safety data sheet when using this product.
- Download the Sample Preparation Guidelines for the Cytek® cFluor® MDSC Kit for
- Download the Acquisition Guidelines for the Cytek® cFluor® MDSC Kit for
- Download the Experiment Template for the Cytek® cFluor® MDSC Kit for
- Download the Assay Tags for the Cytek® cFluor® MDSC Kit for
- Download the Validation Report for the Cytek® cFluor® MDSC Kit
REFERENCES
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For Research Use Only. Not intended for use in diagnostic procedures.
cFluor® B515, cFluor® B548, cFluor® BYG610, cFluor® R685, cFluor® R720, and cFluor® R840 are equivalent to CF®488A, CF®514, RPE-CF®596R, CF®660C, CF®700, and APC-CF®790T respectively, manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.
cFluor® BYG610, cFluor® BYG667, cFluor® BYG710, and cFluor® BYG781 are tandem dyes made with R-PE. cFluor® R840 is a tandem dye made with APC. Caution: Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.