The 5.1H11 monoclonal antibody binds to human CD56, a 120 to 180-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Depending on the cytoplasmic domain, CD56 exists in three main isoforms: NCAM-120, NCAM-140, and NCAM-180¹. CD56, also known as NCAM (Neural Cell Adhesion Molecule), Leu-19 and NKH1, is present on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Aberrant CD56 expression is observed in a range of hematological malignancies such as multiple myeloma and leukemia² as well as in solid tumors such as lung cancer, ovarian cancer, and neuroblastoma³.

PRODUCT DETAILS

Clone: 5.1H11

Reactivity: Human

Format: cFluor^® YG584*

Isotype: Mouse IgG1, κ

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from Light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide
and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Van Acker HH, et al. 2017. Front Immunol. 8:892

2. Crossland DL, et al. 2018. Oncogene. 37(27):3686-3697

3. Seidenfaden R, et al. 2006. Neurochem Int. 49(1):1-11

*cFluor^® YG584 is equivalent to CF^® 568 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

For Research Use Only. Not intended for use in diagnostic procedures.