The mouse monoclonal antibody clone M38 binds to human CD81, a 26 kDa non-glycosylated member of the tetraspanin superfamily (TM4SF). CD81, also known as TAPA-1 (target of an antiproliferative antibody), is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts, and at low levels on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell surface proteins in multimolecular complexes, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.

PRODUCT DETAILS

Clone: M38

Specificity: CD81

Reactivity: Human

Format: cFluor^® V715

Isotype: Mouse IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127-149.
2. Wright M, et al. 1994. Immunol. Today 15:588-594.
3. Tubita et al. 2019. Sci. Rep. 1:11117-11128.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® V715 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

Cytek^® cFluor^® V715 is StarBright^™ Violet 710 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.