cFluor® V670 Anti-Human CD33 (WM53)
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説明
The WM53 monoclonal antibody binds to human CD33, a 67-kDa type I transmembrane glycoprotein in the immunoglobulin family. CD33, also known as Siglec-3, gp67, and p67, is expressed on monocytes, activated T cells, myeloid progenitors, granulocytes, dendritic cells, and mast cells. It is absent on erythrocytes, platelets, and lymphoid cells(1)(2). CD33 binds to sialic acids to act as a sialic acid-dependent cell adhesion molecule. It also contains intracellular tyrosine-based inhibition motifs (ITIMs), suggesting its function to inhibit cellular activity(3).
The antibody was conjugated to a fluorophore and purified by affinity chromatography.
PRODUCT DETAILS
Clone: WM53
Specificity: CD33
Reactivity: Human
Format: cFluor® V670*
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Favaloro EJ, et al. 1987. Dis Markers. 5(4):215-225
- Nakamura Y, et al. 1994. Blood. 83(5):1442-3
- Freeman SD, et al. 1995. Blood. 85(8):2005-2012
- Walter RB, et al. 2007. Traffic. 9(2):267-279
For Research Use Only. Not intended for use in diagnostic procedures.
*cFluor® V670 is StarBright™ Violet 670 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to a company, being subject to certain conditions.